Device

Part:BBa_K176248:Design

Designed by: Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-10-15)


Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3251
    Illegal NheI site found at 3274
    Illegal NheI site found at 4100
    Illegal NheI site found at 4123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2382
    Illegal BglII site found at 3942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5567
    Illegal BsaI.rc site found at 5078


Design Notes

PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Output3: mRFP

Design:

Input1 PoPS generates Output3 mRFP and CI-LVA.

pCI generates LuxI-LVA and TetR-LVA, repressed by CI-LVA.

pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.


Source

K176224 K176226

References